circRNA basic information
circBase ID: hsa_circ_0001368
Name: hsa_circ_KLHL24
Synonym: -
Host Gene: KLHL24
Genomic location(hg19): chr3:183361267-183390272:+
Genomic location(hg38): chr3:183643479-183672484:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric cancer tissue; adjacent nontumor tissue; corresponding paracancer tissues
Cell lines:

HGC-27; MGC-803; AGS; NUGC-3; GES-1; HEK 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: FOXO3
Associated microRNA: miR-6506-5p
Biological function: hsa_circ_0001368 acts as a tumor suppressor in gastric cancer; its knockdown promotes GC cell viability, proliferation, invasion, motility, and tumor growth in vivo.
Molecular mechanism: hsa_circ_0001368 functions as a ceRNA by sponging miR-6506e5p and upregulating the tumor-suppressive target FOXO3.
Biological pathway or process:

proliferation (inhibits); invasion (inhibits); migration (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low hsa_circ_0001368 expression predicts a worse prognosis in GC patients; hsa_circ_0001368 may serve as a potential therapeutic target for GC treatment.
Description:

hsa_circ_0001368 is down-regulated in gastric cancer tissues and cells, and low expression is associated with worse patient prognosis. Functionally, hsa_circ_0001368 suppresses GC proliferation, invasion, migration, and tumor growth. Mechanistically, it acts as a ceRNA sponge for miR-6506e5p/miR-6506-5p to upregulate FOXO3, forming a hsa_circ_0001368/miR-6506e5p/FOXO3 tumor-suppressive axis.
Confidence score:

0.8244
Other information
Title:

Circular RNA hsa_circ_0001368 suppresses the progression of gastric cancer by regulating miR-6506-5p/FOXO3 axis.

Journal: Biochemical and biophysical research communications
Published: 2019
PubMed ID: 30853185
Study type:

combined biological and clinical study
Data availability: -
Code availability: -