circRNA basic information
circBase ID: hsa_circ_0025202
Name: hsa_circ_GAPDH
Synonym: circRNA_0025202
Host Gene: GAPDH
Genomic location(hg19): chr12:6646474-6647162:+
Genomic location(hg38): chr12:6537308-6537996:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
1612
Disease MeSH ID:
-
Disease NCIt ID:
C9335
Disease ICD11 ID:
1047754165
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; adjacent breast tissues; cancer tissues; adjacent normal tissues

Cell lines:

MCF-7; MCF7; MCF7/TR; MCF7/P; T47D; HEK293T; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: FOXO3a, Ago2
Associated microRNA: miR-182-5p
Biological function: hsa_circ_0025202 acts as a tumor suppressor in HR-positive breast cancer, inhibits cell proliferation, colony formation and migration, promotes apoptosis, suppresses xenograft tumor growth, and increases tamoxifen sensitivity.
Molecular mechanism: ceRNA mechanism: hsa_circ_0025202 sponges miR-182-5p and regulates FOXO3a expression and activity, thereby suppressing tumor progression and sensitizing breast cancer cells to tamoxifen.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); apoptosis (promotes); metastasis (inhibits); ceRNA regulation (other); drug resistance (inhibits)

Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis

Clinical significance:

Low hsa_circ_0025202 expression in cancer tissues was negatively correlated with lymphatic metastasis and histological grade, and hsa_circ_0025202 was proposed as a potential biomarker/marker for tamoxifen sensitivity or tamoxifen-resistant breast cancer.

Description:

hsa_circ_0025202 is a GAPDH-derived cytoplasmic circRNA that is down-regulated in tamoxifen-resistant MCF7 cells and HR-positive breast cancer tissues. It suppresses tumor progression and enhances tamoxifen sensitivity by sponging miR-182-5p and regulating FOXO3a. Clinically, its low expression is associated with higher histological grade and lymph node metastasis, suggesting biomarker potential for tamoxifen response.

Confidence score:

0.8685

Other information
Title:

circRNA_0025202 Regulates Tamoxifen Sensitivity and Tumor Progression via Regulating the miR-182-5p/FOXO3a Axis in Breast Cancer.

Journal: Molecular therapy : the journal of the American Society of Gene Therapy
Published: 2019
PubMed ID: 31153828
Study type:

combined biological and clinical study

Data availability: Data S1; Data S2; Data S3; TCGA; Supplemental Information at https://doi.org/10.1016/j.ymthe.2019.05.011
Code availability: -