| Expression pattern: |
UP |
| Associated gene: |
TLR4 |
| Associated microRNA: |
miR-195-5p |
| Biological function: |
circ_0038467 promotes LPS-mediated inhibition of cell viability/proliferation, apoptosis, and inflammatory response in MRC-5 cells; circ_0038467 silencing protects cells from LPS-induced injury. |
| Molecular mechanism: |
circ_0038467 acts as a sponge of miR-195-5p to regulate TLR4 and the NF-kappaB pathway in LPS-induced MRC-5 cells. |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (inhibits); apoptosis (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Annexin V/PI Flow Cytometry; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
providing a theoretical basis for circRNA-directed IP therapy |
| Description: |
circ_0038467 is up-regulated in LPS-induced human MRC-5 cells, an in vitro model related to infantile pneumonia. It aggravates LPS-induced cell injury by suppressing proliferation/cell viability and promoting apoptosis and inflammatory cytokine production, acting through the miR-195-5p/TLR4 axis and NF-kappaB pathway. |
| Confidence score: |
0.7204 |