circRNA basic information
circBase ID: hsa_circ_0001495
Name: hsa_circ_CCNB1
Synonym: Circular RNA_0001495
Host Gene: CCNB1
Genomic location(hg19): chr5:68470703-68471364:+
Genomic location(hg38): chr5:69174876-69175537:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCa tissues; paired adjacent normal tissues; tumor tissue; lung
Cell lines:

T24; RT4; J82; 5637; UM-UC-3; HT-1376; TCCSUP; SV-HUC-1; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Robo1, Ago2
Associated microRNA: miR-527
Biological function: Promotes proliferation, migration and invasion of BCa cells in vitro, and promotes tumor growth and metastasis in vivo.
Molecular mechanism: circ_0001495 acts as a miRNA sponge for miR-527, inhibits miR-527 activity, and increases Robo1 expression to promote BCa progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0001495 expression is correlated with higher grades and T stages of BCa and predicts poor prognosis in BCa patients.
Description:

circ_0001495 is up-regulated in bladder cancer tissues and cells and is associated with higher tumor grade, advanced T stage, and poorer overall survival. Functionally, it promotes BCa cell proliferation, migration, invasion, tumor growth, and metastasis by sponging miR-527 and increasing Robo1 expression.
Confidence score:

0.8824
Other information
Title:

Circular RNA_0001495 increases Robo1 expression by sponging microRNA-527 to promote the proliferation, migration and invasion of bladder cancer cells.

Journal: Carcinogenesis
Published: 2021
PubMed ID: 34021307
Study type:

combined biological and clinical study
Data availability: TCGA
Code availability: -