circRNA basic information
circBase ID: hsa_circ_0000337
Name: hsa_circ_PPFIA1
Synonym: circ_0000337
Host Gene: PPFIA1
Genomic location(hg19): chr11:70200406-70202360:+
Genomic location(hg38): chr11:70354300-70356254:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

U2OS; HOS; 143B; MG-63; NHOst; hFOB1.19
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: BACH1, Ago2
Associated microRNA: miR-4458
Biological function: promotes OS cell proliferation and migration, inhibits apoptosis, and facilitates OS progression
Molecular mechanism: circ_0000337 acts as a ceRNA by sponging miR-4458 to upregulate BACH1 expression
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Western Blot
Clinical significance:

offering rational therapeutic target for OS
Description:

circ_0000337 is upregulated in osteosarcoma cell lines and promotes OS cell proliferation and migration while suppressing apoptosis. Mechanistically, it functions as a miR-4458 sponge to increase BACH1 expression, forming a circ_0000337/miR-4458/BACH1 regulatory pathway that facilitates OS progression and may provide a therapeutic target.
Confidence score:

0.6528
Other information
Title:

Circular RNA circ_0000337 contributes to osteosarcoma via the miR-4458/BACH1 pathway.

Journal: Cancer biomarkers : section A of Disease markers
Published: 2020
PubMed ID: 32390598
Study type:

biological research
Data availability: -
Code availability: -