| Expression pattern: |
UP |
| Associated gene: |
SEPT2, ERK1/2, AGO2 |
| Associated microRNA: |
miR-497 |
| Biological function: |
Promotes PCa cell proliferation and invasion and reduces cell death; EV-derived circ_SLC19A1 can be transferred into recipient PCa cells and regulate their biological functions. |
| Molecular mechanism: |
EV-derived circ_SLC19A1 acts as a miR-497 sponge to relieve miR-497-mediated suppression of SEPT2, thereby activating the downstream ERK1/2 pathway. |
| Biological pathway or process: |
ERK (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
EV-derived circ_SLC19A1 may be an important target for PCa prevention and treatment. |
| Description: |
circ_SLC19A1 is up-regulated in prostate cancer cells and their extracellular vesicles. EV-derived circ_SLC19A1 promotes PCa cell proliferation and invasion and suppresses cell death by sponging miR-497, increasing SEPT2 expression, and activating ERK1/2 signaling. The study suggests that EV-derived circ_SLC19A1 may be a therapeutic target for PCa prevention and treatment. |
| Confidence score: |
0.6853 |