| Expression pattern: |
UP |
| Associated gene: |
AGO2, AKT, FOXO1, mTOR, Bcl-2, Bax, cleaved-PARP, MMP-9, TIMP-1 |
| Associated microRNA: |
miR-99a |
| Biological function: |
circGFRA1 promotes glioma cell viability, proliferation, colony formation and migration, and inhibits apoptosis; knockdown circGFRA1 suppresses these malignant phenotypes and enhances apoptosis. |
| Molecular mechanism: |
circGFRA1 acts as a miR-99a sponge; silencing circGFRA1 upregulates miR-99a and blocks AKT/FOXO1 and mTOR pathways. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; TUNEL; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circGFRA1 served as a biomarker and novel therapeutic candidate for glioma. |
| Description: |
circGFRA1 is upregulated in human glioma tissues. In U251 glioma cells, circGFRA1 knockdown suppresses viability, proliferation, colony formation and migration while promoting apoptosis. Mechanistically, circGFRA1 functions as a miR-99a sponge and modulates AKT/FOXO1 and mTOR pathway activity. |
| Confidence score: |
0.705 |