| Expression pattern: |
DN |
| Associated gene: |
BCL-2 |
| Associated microRNA: |
miR-544a-3p |
| Biological function: |
Suppresses gastric cancer progression by inhibiting proliferation, migration, invasion, and cell-cycle progression, promoting apoptosis, and inhibiting autophagy. |
| Molecular mechanism: |
Acts as a cytoplasmic sponge of miR-544a-3p to regulate the miR-544a-3p/BCL-2 axis, thereby inhibiting autophagosome formation and affecting malignant phenotypes. |
| Biological pathway or process: |
autophagy (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
Its expression correlated with tumor invasion depth (tumor depth). |
| Description: |
hsa_circ_0009109 (circDCAF6_003) is down-regulated in gastric cancer tissues and cell lines and acts as a tumor suppressor. It mainly localizes to the cytoplasm, sponges miR-544a-3p, upregulates BCL-2, and inhibits autophagy, thereby restraining proliferation, migration/invasion, and tumor growth. |
| Confidence score: |
0.8072 |