| Expression pattern: |
UP |
| Associated gene: |
CDC25A, Ago2 |
| Associated microRNA: |
miR-339-5p |
| Biological function: |
Promotes ESCC cell proliferation, migration and invasion; supports cell-cycle progression; promotes xenograft tumor growth; serves as a less-invasive diagnostic and prognostic biomarker. |
| Molecular mechanism: |
hsa circ 0004771 acts as a miR-339-5p sponge and positively regulates CDC25A, thereby promoting ESCC progression partly through CDC25A signaling and cell-cycle progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression correlates with heavier tumor burden, T grade, vascular invasion, shorter OS and DFS, and poorer prognosis; plasma hsa circ 0004771 has diagnostic value with ROC AUC 0.816. |
| Description: |
hsa_circ_0004771 is upregulated in ESCC plasma and tumor tissues and high plasma expression predicts poorer OS and DFS. Functionally, it promotes ESCC proliferation, migration, invasion and in vivo tumor growth. Mechanistically, it mainly localizes in the cytoplasm and acts through a miR-339-5p/CDC25A axis, with evidence suggesting tumor-derived exosomal contribution to its plasma abundance. |
| Confidence score: |
0.8867 |