| Expression pattern: |
UP |
| Associated gene: |
AGO2, CDK6 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
Promotes osteosarcoma cell proliferation, colony formation, cell cycle progression from G1 to S phase, tumor growth, and osteosarcoma progression; knockdown suppresses OS cell growth and arrests cell cycle progression. |
| Molecular mechanism: |
circ_ARF3 acts as a ceRNA/miRNA sponge for miR-1299, relieving miR-1299-mediated CDK6 downregulation and maintaining CDK6 expression in osteosarcoma. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_ARF3 can be used as a novel biomarker for osteosarcoma diagnosis or molecular target for future osteosarcoma treatment. |
| Description: |
circ_ARF3 is up-regulated in osteosarcoma cell lines and tumor tissues. It promotes osteosarcoma cell proliferation, colony formation, G1/S cell-cycle transition, and xenograft tumor growth by sponging miR-1299 and maintaining CDK6 expression. The study suggests circ_ARF3 as a potential biomarker or therapeutic target for osteosarcoma. |
| Confidence score: |
0.8107 |