| Expression pattern: |
UP |
| Associated gene: |
ADAM10 / ADAM metallopeptidase domain 10 / PTPRA |
| Associated microRNA: |
miR-1227-3p |
| Biological function: |
circ_0114876 facilitated OA progression in an LPS-induced chondrocyte model; circ_0114876 knockdown promoted C28/I2 cell proliferation and suppressed apoptosis, inflammatory response, and extracellular matrix degradation. |
| Molecular mechanism: |
circ_0114876 acts as a ceRNA for miR-1227-3p to upregulate ADAM10, thereby regulating proliferation, apoptosis, inflammatory response and ECM degradation in OA chondrocytes. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0114876 may be a therapeutic target and diagnostic biomarker of OA. |
| Description: |
circ_0114876 is up-regulated in OA tissues and LPS-induced human chondrocytes. Functionally, circ_0114876 promotes OA-like chondrocyte injury by inhibiting proliferation and enhancing apoptosis, inflammation, and ECM degradation. Mechanistically, circ_0114876 functions through the miR-1227-3p/ADAM10 ceRNA axis and may represent a potential therapeutic or diagnostic target for OA. |
| Confidence score: |
0.7945 |