| Expression pattern: |
UP |
| Associated gene: |
COL5A1 |
| Associated microRNA: |
miR-29a-3p, miR-29b-3p |
| Biological function: |
circACAP2 promotes breast cancer cell viability, proliferation, migration, invasion, motility, metastasis and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circACAP2 acts as a ceRNA/miRNA sponge for miR-29a-3p and miR-29b-3p, thereby modulating COL5A1 expression to promote breast cancer progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; TUNEL; Transwell Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circACAP2 could be a potential biomarker and therapeutic target for diagnosis and treatment of breast cancer. |
| Description: |
circACAP2 is up-regulated in breast cancer tissues and cell lines. It promotes breast cancer proliferation, migration, invasion, metastasis and xenograft tumor growth while suppressing apoptosis, mainly by sponging miR-29a-3p and miR-29b-3p to regulate COL5A1. The study suggests circACAP2 may serve as a potential diagnostic and therapeutic biomarker for breast cancer. |
| Confidence score: |
0.7525 |