| Expression pattern: |
UP |
| Associated gene: |
SATB1 |
| Associated microRNA: |
miR-153-3p |
| Biological function: |
Promotes ESCC cell proliferation, cell cycle progression, migration, invasion and EMT, and inhibits apoptosis; triptolide suppresses these malignant phenotypes partly by reducing circNOX4. |
| Molecular mechanism: |
circNOX4 acts as a miRNA sponge for miR-153-3p to de-repress SATB1 (ceRNA mechanism), forming a circNOX4/miR-153-3p/SATB1 axis affected by triptolide. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Actinomycin D / DRB Stability Assay; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circNOX4 is up-regulated in ESCC and promotes malignant phenotypes (proliferation, cell cycle progression, migration/invasion and EMT) while inhibiting apoptosis. Mechanistically, circNOX4 sponges miR-153-3p to upregulate SATB1; triptolide suppresses ESCC partly by downregulating circNOX4 and inhibiting the circNOX4/miR-153-3p/SATB1 axis. |
| Confidence score: |
0.8443 |