| Expression pattern: |
UP |
| Associated gene: |
KCNH1 |
| Associated microRNA: |
miR-1225-3p |
| Biological function: |
Promotes OS cell proliferation, colony formation, migration, invasion, glycolysis, and tumor growth in vivo. |
| Molecular mechanism: |
circ_0016347 functions as a sponge of miR-1225-3p to upregulate KCNH1 expression, thereby promoting OS progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
might provide a promising biomarker for OS therapy; might provide a novel therapeutic strategy for OS patients |
| Description: |
circ_0016347 is up-regulated in osteosarcoma tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion, glycolysis, and xenograft tumor growth by sponging miR-1225-3p and increasing KCNH1 expression, suggesting potential therapeutic relevance in OS. |
| Confidence score: |
0.8001 |