| Expression pattern: |
UP |
| Associated gene: |
ACTG1, P4HB |
| Associated microRNA: |
- |
| Biological function: |
enhances EMT and promotes BLCA cell proliferation, migration, invasion, tumor growth and metastasis; promotes M2 macrophage polarization/infiltration |
| Molecular mechanism: |
ACTG1 promotes alternative splicing of P4HB transcript to generate circ_0046263; circ_0046263 mediates EMT-related metastasis phenotypes and modulates tumor immune microenvironment via M2 macrophage polarization |
| Biological pathway or process: |
EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); immune regulation (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Microarray |
| Clinical significance: |
- |
| Description: |
circ_0046263 (derived from P4HB) is up-regulated in BLCA cells and functions downstream of ACTG1-driven splicing to promote EMT, proliferation, migration, invasion, and metastasis. Re-expression of circ_0046263 rescues the anti-tumor effects caused by ACTG1 knockdown in vitro and in vivo, and it contributes to M2 macrophage polarization/infiltration. |
| Confidence score: |
0.7375 |