circRNA basic information
circBase ID: hsa_circ_0000423
Name: hsa_circ_PPP1R12A
Synonym: circPPP1R12A / Circ_0000423
Host Gene: PPP1R12A
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0021063
MONDO name: malignant colon neoplasm
Disease details: colon cancer
Disease DO ID:
219
Disease MeSH ID:
-
Disease NCIt ID:
C9242
Disease ICD11 ID:
1265576634
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tumor tissues; adjacent normal tissues; xenograft tumor tissues

Cell lines:

NCM460; SW48; HCT15

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CTNNB1
Associated microRNA: miR-375
Biological function: circPPP1R12A promotes CC cell proliferation, cell cycle progression, migration, invasion and tumor growth, and inhibits apoptosis.
Molecular mechanism: circPPP1R12A acts as a ceRNA by sponging miR-375, thereby positively regulating CTNNB1 expression and promoting CC progression.
Biological pathway or process:

ceRNA regulation (promotes); Wnt/beta-catenin (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Wound Healing Assay; Transwell Assay; In Vivo Animal Model

Clinical significance:

circPPP1R12A might be a potential biomarker and therapeutic target for CC.

Description:

circPPP1R12A is up-regulated in colon cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, cell cycle progression, migration, invasion and xenograft tumor growth while suppressing apoptosis, mainly through the circPPP1R12A/miR-375/CTNNB1 ceRNA axis.

Confidence score:

0.7183

Other information
Title:

CircPPP1R12A promotes the progression of colon cancer through regulating CTNNB1 via sponging miR-375.

Journal: Anti-cancer drugs
Published: 2021
PubMed ID: 33595945
Study type:

combined biological and clinical study

Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -