| Expression pattern: |
UN |
| Associated gene: |
Wnt-3, Wnt-5a |
| Associated microRNA: |
- |
| Biological function: |
promotes cell proliferation; promotes invasion |
| Molecular mechanism: |
Predicted ceRNA (circRNA-miRNA-mRNA) involvement with WNT signaling-related target genes (Wnt-3/5a). |
| Biological pathway or process: |
Wnt/beta-catenin (other); proliferation (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Transfection; MTT; Transwell Assay; Colony Formation Assay; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Sanger Sequencing |
| Clinical significance: |
Included in 60 CSF-circRNAs that were identified and associated with shorten overall survival; circ_0008604 was only identified in CSF from lung cancer patients in the validation cohort. |
| Description: |
hsa_circ_0008604 (RHOT1-derived) was a WNT-pathway-associated CSF circRNA and was detected only in CSF from lung cancer BM patients in an independent validation set. Knockdown experiments in A549 implied it supports proliferation/invasion, and network analysis linked it to WNT signaling targets (Wnt-3/5a). |
| Confidence score: |
0.7718 |