| Expression pattern: |
DN |
| Associated gene: |
DUSP9, ERK1/2, JNK |
| Associated microRNA: |
miR-1233-3p |
| Biological function: |
Promotes apoptosis and inhibits proliferation of A549 cells; acts as a tumor suppressor in NSCLC. |
| Molecular mechanism: |
hsa_circ_0004050 functions as a molecular sponge of miR-1233-3p, increases DUSP9 protein expression, and suppresses the ERK/JNK signaling pathway. |
| Biological pathway or process: |
MAPK (inhibits); ERK (inhibits); apoptosis (promotes); proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Decreased expression is associated with TNM stage, tumor size and tumor metastasis; high expression is positively correlated with longer survival time; may be used as a biomarker for diagnosis or treatment of NSCLC. |
| Description: |
hsa_circ_0004050 is down-regulated in NSCLC tissues and A549 cells and acts as a tumor suppressor. It sponges miR-1233-3p to increase DUSP9 expression, thereby inhibiting ERK/JNK signaling, suppressing proliferation and promoting apoptosis. Clinically, higher hsa_circ_0004050 expression is associated with longer survival and may have diagnostic or therapeutic biomarker potential. |
| Confidence score: |
0.8057 |