circRNA basic information
circBase ID: hsa_circ_0000514
Name: -
Synonym: circular RNA 0000514 / circ_0000514
Host Gene: -
Genomic location(hg19): chr14:20811305-20811436:-
Genomic location(hg38): chr14:20343146-20343277:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancer tissues; adjacent tissue samples; BC tissues; adjacent tissues
Cell lines:

MCF-7; BT-474; MDA-MB-231; SK-BR-3; HBL-100
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CXCL10
Associated microRNA: miR-296-5p
Biological function: promotes BC cell proliferation, migration, and invasion
Molecular mechanism: circ_0000514 acts as a ceRNA by adsorbing miR-296-5p to up-regulate CXCL10 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; BrdU; Transwell Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

circ_0000514 expression was associated with tumor size and TNM stage; it had potential to be a diagnostic and prognostic biomarker for BC
Description:

circ_0000514 is up-regulated in breast cancer tissues and cell lines. It promotes malignant biological behaviors of breast cancer cells, including proliferation, migration, and invasion, by adsorbing miR-296-5p and increasing CXCL10 expression. Its expression is associated with tumor size and TNM stage, suggesting potential biomarker value.
Confidence score:

0.7404
Other information
Title:

Circ_0000514 promotes breast cancer progression by regulating the miR-296-5p/CXCL10 axis.

Journal: Journal of biochemistry
Published: 2022
PubMed ID: 34528678
Study type:

combined biological and clinical study
Data availability: GSE101123
Code availability: -