| Expression pattern: |
UP |
| Associated gene: |
MAFK, SIRT1, Keap1, PD-L1, TIM3 |
| Associated microRNA: |
- |
| Biological function: |
circRPPH1 promotes LUAD cell proliferation and tumor growth, maintains mitochondrial stability, inhibits cellular pyroptosis, senescence and apoptosis, and is associated with metastasis and poor prognosis. |
| Molecular mechanism: |
circRPPH1 binds to the transcription factor MAFK in the nucleus, promotes MAFK-mediated SIRT1 transcription/expression and activates the MAFK-Keap1 signaling pathway, thereby inhibiting caspase-3/GSDME-associated cellular pyroptosis and promoting LUAD progression. |
| Biological pathway or process: |
pyroptosis (inhibits); proliferation (promotes); apoptosis (inhibits); mitochondrial function (other); p53 signaling (inhibits); immune regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; TUNEL; IF (Immunofluorescence); Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circRPPH1 was associated with poor prognosis, shorter survival, lymph node metastasis, distant metastasis, and serum tumor markers CEA, NSE, and CA199 in LUAD patients. |
| Description: |
circRPPH1/hsa_circ_0000515 is up-regulated in LUAD and high expression is linked to poor survival and metastasis-related clinical features. Mechanistically, nuclear circRPPH1 binds MAFK to enhance SIRT1 transcription/expression, suppressing cellular pyroptosis and promoting LUAD cell growth in vitro and in xenograft models. |
| Confidence score: |
0.8981 |