| Expression pattern: |
DN |
| Associated gene: |
MAPK6 |
| Associated microRNA: |
- |
| Biological function: |
CircRNA_100395 acts as an anti-cancer gene in breast carcinoma; overexpression weakens proliferative, colony formation and migratory abilities of breast carcinoma cells. |
| Molecular mechanism: |
CircRNA_100395 directly targets MAPK6 and negatively regulates MAPK6 expression, thereby alleviating malignant progression of breast carcinoma. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircRNA_100395 level is negatively correlated with tumor staging and tumor size of breast carcinoma and may serve as a potential biomarker and therapeutic target. |
| Description: |
CircRNA_100395 is downregulated in breast carcinoma tissues and cell lines. It functions as an anti-cancer circRNA by suppressing breast carcinoma cell proliferation, colony formation and migration, at least partly through targeting and negatively regulating MAPK6. Its reduced expression is clinically associated with larger tumor size and more advanced tumor staging, supporting potential biomarker and therapeutic relevance. |
| Confidence score: |
0.6536 |