| Expression pattern: |
UP |
| Associated gene: |
YY1, CTNNB1 |
| Associated microRNA: |
miR-4262, miR-185-5p |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, stemness, tumor growth and metastasis; inhibits apoptosis. |
| Molecular mechanism: |
circAGFG1 acts as a ceRNA by sponging miR-4262 and miR-185-5p, upregulating YY1, which transcriptionally activates CTNNB1 and activates Wnt/beta-catenin signaling. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); metastasis (promotes); stemness (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; TUNEL; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
circAGFG1 expression in tumor tissues from CRC patients with liver metastasis was higher than that in those without liver metastasis. |
| Description: |
circAGFG1 is upregulated in colorectal cancer cells and tissues and promotes proliferation, migration/invasion, stemness, tumor growth and metastasis while inhibiting apoptosis. Mechanistically, it sponges miR-4262 and miR-185-5p to increase YY1, which transcriptionally activates CTNNB1 and thereby activates Wnt/beta-catenin signaling. |
| Confidence score: |
0.8785 |