circRNA basic information
circBase ID: -
Name: -
Synonym: circRNA-100338
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum; plasma exosomes; HCC tissues; lung metastatic nodules
Cell lines:

L02; Hep3B; HLE; Huh7; BEL7402; SMMC7721; MHCC97L; MHCC97H; HCCLM3; HCCLM6; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MMP9, NOVA2, FUS, IF2B1, IF2B3, IF2B2, RBM39, RBM14, PAIRB, EWS, NOVA1, RBM26, RBM27, RBM10, RBM15, T2FA, HLTF, GTF2I, T2EA, BCLF1, DCP1A, p53, HDAC1, HDAC2, HPF1, VE-Cadherin, ZO-1
Associated microRNA: -
Biological function: promotes HCC invasiveness and metastasis; promotes angiogenesis, proliferation, permeability and vasculogenic mimicry in HUVECs
Molecular mechanism: Exosome-mediated transfer to recipient HUVECs; binds multiple RNA-binding proteins and other proteins (including NOVA2) based on RNA pull-down and mass spectrometry; regulates VE-Cadherin and ZO-1 protein levels; associated with increased MMP9
Biological pathway or process:

angiogenesis (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; Transwell Assay; MTT; Tube Formation Assay; RNA Pull-Down; Western Blot; ELISA; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Persistent high expression of serum exosomal circRNA-100,338 after curative hepatectomy may indicate pulmonary metastasis risk and poor survival; Post/Pre ratio used as prognostic indicator
Description:

Exosomal circRNA-100,338 is up-regulated in highly metastatic HCC cells and their exosomes and promotes HCC invasion and metastasis. After transfer to recipient HUVECs, it enhances endothelial proliferation, angiogenesis, permeability and VM formation, partly associated with altered VE-Cadherin/ZO-1 and increased MMP9, and it binds multiple proteins (including NOVA2). Serum exosomal circRNA-100,338 dynamics after hepatectomy are associated with pulmonary metastasis risk and poorer survival.
Confidence score:

0.7132
Other information
Title:

Exosomal circRNA-100338 promotes hepatocellular carcinoma metastasis via enhancing invasiveness and angiogenesis.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2020
PubMed ID: 31973767
Study type:

combined biological and clinical study
Data availability: All remaining data are available within the article or available from the authors upon request.
Code availability: -