| Expression pattern: |
UP |
| Associated gene: |
TXNIP |
| Associated microRNA: |
miR-370 |
| Biological function: |
Circ-BANP promotes ox-LDL-induced endothelial cell injury in HUVECs, including reduced cell viability, increased pro-inflammatory cytokine production and apoptosis, and suppressed migration, invasion and tube formation; circ-BANP silencing alleviates these injuries. |
| Molecular mechanism: |
Circ-BANP functions as a cytoplasmic ceRNA-like regulator that directly binds miR-370 and thereby controls TXNIP expression through the circ-BANP/miR-370/TXNIP axis. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); migration (inhibits); invasion (inhibits); angiogenesis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; ELISA; Western Blot |
| Clinical significance: |
circ-BANP is up-regulated in AS blood and is proposed as a potential therapeutic target for endothelial cell dysfunction in AS. |
| Description: |
This study identifies circ-BANP/hsa_circ_0040824 as an up-regulated human circRNA in AS blood and ox-LDL-treated endothelial cells. Circ-BANP aggravates ox-LDL-induced HUVEC injury by directly targeting miR-370 and relieving miR-370-mediated repression of TXNIP, while circ-BANP silencing improves endothelial cell viability, reduces inflammation and apoptosis, and restores migration, invasion and tube formation. |
| Confidence score: |
0.742 |