| Expression pattern: |
UP |
| Associated gene: |
ANXA2, ATG5, ATG7, p-mTOR |
| Associated microRNA: |
miR-6881-3p |
| Biological function: |
Promotes autophagy, proliferation, invasion, xenograft tumor growth, and metastasis in epithelial ovarian cancer. |
| Molecular mechanism: |
CircEEF2 promotes autophagy through a ceRNA mechanism by sponging miR-6881-3p to increase ATG5 and ATG7, and through direct binding to ANXA2 to inhibit p-mTOR activation. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); invasion (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); PI3K/AKT/mTOR (inhibits); other pathway/process (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Transfection; EdU Staining; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); RNA-seq |
| Clinical significance: |
CircEEF2 expression was related to FIGO stage and tumor grade, and may serve as a potential biomarker and target for epithelial ovarian cancer. |
| Description: |
CircEEF2 is up-regulated in epithelial ovarian cancer tissues and is associated with advanced FIGO stage and higher tumor grade. Functionally, it promotes autophagy-driven proliferation, invasion, xenograft tumor growth, and metastasis, mainly by sponging miR-6881-3p to increase ATG5/ATG7 and by binding ANXA2 to suppress p-mTOR activation. The study suggests circEEF2 as a potential biomarker and therapeutic target for ovarian cancer. |
| Confidence score: |
0.8502 |