circRNA basic information
circBase ID: hsa_circ_0047814
Name: hsa_circ_ZNF532
Synonym: circ_0047814 / cZNF532
Host Gene: ZNF532
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005266
MONDO name: diabetic retinopathy
Disease details: diabetic retinopathy / DR
Disease DO ID:
8947
Disease MeSH ID:
D003930
Disease NCIt ID:
C34538
Disease ICD11 ID:
1006882070
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

retinal vessels; retinas; vitreous humor; vitreous samples

Cell lines:

Human retinal pericytes (ACBRI-183); HRVECs (ACBRI-181); HEK293T

In vivo animal model:

genetically engineered animal model; other disease animal model

circRNA-disease information
Expression pattern:
UP
Associated gene: SP1, NG2, CSPG4, LOXL2, CDK2
Associated microRNA: miR-29a-3p, miR-498, miR-758
Biological function: cZNF532 maintains pericyte viability, proliferation, differentiation and recruitment toward endothelial cells, protects pericytes from diabetic stress-induced apoptosis, and ameliorates diabetes-induced retinal pericyte degeneration and vascular dysfunction.
Molecular mechanism: SP1 activates cZNF532 transcription under diabetic stress; cytoplasmic cZNF532 acts as a miR-29a-3p sponge, reducing miR-29a-3p activity and increasing NG2/CSPG4, LOXL2, and CDK2 expression.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); inflammation (inhibits); other pathway/process (inhibits)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; ChIP / ChIP-seq; Transfection; MTT; Cell Cycle Assay; IF (Immunofluorescence); In Vivo Animal Model; ELISA

Clinical significance:

cZNF532 expression is upregulated in the vitreous of patients with DME, PDR, or NVI, correlates with disease severity, and is proposed as a promising biomarker for the diagnosis and prognosis of DR.

Description:

cZNF532 is a high glucose- and diabetic stress-induced circRNA derived from ZNF532 that is upregulated in pericytes, diabetic mouse retinal vessels, and vitreous from patients with diabetic retinopathy. It protects retinal pericyte function and vascular integrity through a cZNF532/miR-29a-3p/NG2-LOXL2-CDK2 ceRNA mechanism, and its vitreous expression correlates with DR severity.

Confidence score:

0.8237

Other information
Title:

Circular RNA-ZNF532 regulates diabetes-induced retinal pericyte degeneration and vascular dysfunction.

Journal: The Journal of clinical investigation
Published: 2020
PubMed ID: 32343678
Study type:

combined biological and clinical study

Data availability: GSE14367; Supplemental Data Set 1; Supplemental Data Set 2; Supplemental Data Set 3
Code availability: -