circRNA basic information
circBase ID: hsa_circ_0005728
Name: hsa_circ_UBE2D2
Synonym: CircUBE2D2
Host Gene: UBE2D2
Genomic location(hg19): chr5:138979956-138994551:+
Genomic location(hg38): chr5:139600371-139614966:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paracancerous tissues
Cell lines:

BT-549; SUM-159; MDA-MB-231; MDA-MB-468; HCC38; MCF-10A; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CDCA3, Ago2
Associated microRNA: miR-512-3p
Biological function: Promotes TNBC cell proliferation, migration, invasion/metastasis, tumor growth in vivo, and doxorubicin resistance; inhibits doxorubicin-induced apoptosis when highly expressed.
Molecular mechanism: Acts as a cytoplasmic miRNA sponge for miR-512-3p, thereby relieving miR-512-3p-mediated repression of CDCA3 and increasing CDCA3 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Survival Analysis
Clinical significance:

High circUBE2D2 expression is associated with advanced TNM stage, lymph node metastasis, and poorer overall survival (adverse prognosis) in TNBC patients.
Description:

circUBE2D2 (hsa_circ_0005728) is up-regulated in TNBC and mainly localized in the cytoplasm. It promotes malignant phenotypes (proliferation, migration/invasion, tumor growth) and doxorubicin chemoresistance by sponging miR-512-3p, thereby up-regulating CDCA3.
Confidence score:

0.8591
Other information
Title:

CircUBE2D2 (hsa_circ_0005728) promotes cell proliferation, metastasis and chemoresistance in triple-negative breast cancer by regulating miR-512-3p/CDCA3 axis.

Journal: Cancer cell international
Published: 2020
PubMed ID: 32944002
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -