circRNA basic information
circBase ID: hsa_circ_0008035
Name: hsa_circ_EXT1
Synonym: Circ_0008035
Host Gene: EXT1
Genomic location(hg19): chr8:118830673-118849440:-
Genomic location(hg38): chr8:117818434-117837201:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissues; xenograft tumors
Cell lines:

AGS; HGC-27; GES-1; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SMAD2
Associated microRNA: miR-429
Biological function: Promotes GC cell proliferation, cell cycle progression, angiogenesis, migration and invasion; inhibits apoptosis; promotes xenograft tumor growth.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-429 to increase SMAD2 expression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Transfection; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Tube Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; Clinical Sample Validation; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

circ_0008035 (from host gene EXT1) is up-regulated in gastric cancer tissues and cell lines and promotes malignant phenotypes (proliferation, cell-cycle progression, migration/invasion, angiogenesis) while inhibiting apoptosis. Mechanistically, it sponges miR-429 to increase SMAD2 expression, and circ_0008035 knockdown suppresses xenograft tumor growth.
Confidence score:

0.7488
Other information
Title:

Circ_0008035 Promotes Gastric Cancer Development via the miR-429/SMAD2 Cascade.

Journal: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology
Published: 2024
PubMed ID: 39412408
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available on request from the corresponding author.
Code availability: -