| Expression pattern: |
UP |
| Associated gene: |
IKBKB, AGO2 |
| Associated microRNA: |
miR-218-5p, miR-1273e |
| Biological function: |
promotes OS cell proliferation, migration, invasion and tumor growth |
| Molecular mechanism: |
ceRNA/miRNA sponge: circ_0028171 sponges miR-218-5p to increase IKBKB expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
potential novel marker for early diagnosis of OS |
| Description: |
circ_0028171 is up-regulated in osteosarcoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes osteosarcoma proliferation, migration, invasion and in vivo tumor growth by sponging miR-218-5p, thereby increasing IKBKB expression. |
| Confidence score: |
0.8228 |