circRNA basic information
circBase ID: hsa_circ_0000936
Name: hsa_circ_SHKBP1
Synonym: circ-SHKBP1
Host Gene: SHKBP1
Genomic location(hg19): chr19:41089303-41089623:+
Genomic location(hg38): chr19:40583397-40583717:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
-
Disease MeSH ID:
D005910
Disease NCIt ID:
C3059
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; adjacent normal tissues

Cell lines:

U251; U87; U251/TMZ-R; U87/TMZ-R

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: miR-1294
Biological function: hsa_circ_0000936 promotes temozolomide resistance in glioma cells; knockdown of hsa_circ_0000936 increases TMZ sensitivity and increases apoptosis in TMZ-resistant glioma cell lines.
Molecular mechanism: hsa_circ_0000936 acts as a molecular sponge for miR-1294 to confer temozolomide resistance in glioma cells.
Biological pathway or process:

apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry

Clinical significance:

hsa_circ_0000936 is upregulated in glioma tissues and higher in tissues from TMZ-resistant patients; it may be a potential target for overcoming TMZ resistance in glioma.

Description:

hsa_circ_0000936 is upregulated in glioma tissues, especially in tissues from patients resistant to temozolomide, and is also elevated in TMZ-resistant glioma cell lines. Functionally, hsa_circ_0000936 promotes TMZ resistance and suppresses apoptosis under TMZ treatment by sponging miR-1294; its knockdown sensitizes resistant glioma cells to TMZ.

Confidence score:

0.6369

Other information
Title:

Downregulation of hsa_circ_0000936 sensitizes resistant glioma cells to temozolomide by sponging miR-1294.

Journal: Journal of biosciences
Published: 2020
PubMed ID: 32975228
Study type:

combined biological and clinical study

Data availability: -
Code availability: -