| Expression pattern: |
UP |
| Associated gene: |
DDIT4 |
| Associated microRNA: |
miR-495-3p |
| Biological function: |
promotes MM cell proliferation, reduces apoptosis, and accelerates cell cycle progression; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circXPO1 sponges miR-495-3p to increase DDIT4 expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Survival Analysis; RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
circXPO1 had diagnostic value for MM (AUC 0.815) and high circXPO1 expression was associated with poor survival; positively related to blood beta2-MG levels and ISS stage |
| Description: |
circXPO1 is up-regulated in multiple myeloma bone marrow and MM cell lines and is associated with worse survival. Functionally, circXPO1 promotes proliferation and cell-cycle progression and inhibits apoptosis, at least partly by sponging miR-495-3p to upregulate DDIT4, and its silencing suppresses xenograft tumor growth. |
| Confidence score: |
0.8718 |