| Expression pattern: |
UP |
| Associated gene: |
CREB1, HK2, LDHA |
| Associated microRNA: |
miR-383 |
| Biological function: |
promotes proliferation and glycolysis; inhibits apoptosis; accelerates tumor growth |
| Molecular mechanism: |
circ_0136666 sponges miR-383 to up-regulate CREB1, thereby increasing glycolysis-related proteins HK2 and LDHA |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circ_0136666 expression was positively associated with tumor size, TNM stages, lymphatic metastasis and distant metastasis in CRC patients |
| Description: |
circ_0136666 is up-regulated in colorectal cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes CRC cell proliferation and glycolysis and inhibits apoptosis by sponging miR-383 to up-regulate CREB1, leading to increased HK2 and LDHA and enhanced tumor growth in xenografts. |
| Confidence score: |
0.6521 |