| Expression pattern: |
UP |
| Associated gene: |
HDGF / HDGF mRNA |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
promotes glycolysis and proliferation of NSCLC cells; promotes tumor growth in vivo |
| Molecular mechanism: |
circMAGI3 acts as a miR-515-5p sponge to relieve HDGF expression, thereby accelerating glycolysis of NSCLC |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Survival Analysis; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; In Vivo Animal Model |
| Clinical significance: |
circMAGI3 was closely correlated with unfavorable outcome for NSCLC patients; higher circMAGI3 indicated lower survival rate |
| Description: |
circMAGI3 is a human MAGI3-derived circRNA that is up-regulated in NSCLC tissues and cell lines and is associated with poor survival. It promotes NSCLC glycolysis, proliferation, and xenograft tumor growth by sponging miR-515-5p and relieving HDGF expression. |
| Confidence score: |
0.8318 |