| Expression pattern: |
UP |
| Associated gene: |
SLC7A11, c-myc, bcl-xl |
| Associated microRNA: |
miR-485-5p |
| Biological function: |
Promotes LUAD cell proliferation and tumor growth, inhibits apoptosis and ferroptosis, and enhances malignant progression. |
| Molecular mechanism: |
circ_0070440 acts as a miR-485-5p sponge to increase SLC7A11 expression, thereby suppressing ferroptosis-related phenotypes and promoting LUAD progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ferroptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
The circ_0070440/miR-485-5p/SLC7A11 axis may serve as potential biomarkers for clinical detection in LUAD. |
| Description: |
hsa_circ_0070440 is up-regulated in LUAD tissues and cells. It promotes LUAD cell proliferation and tumor growth while inhibiting apoptosis and ferroptosis, at least partly by sponging miR-485-5p to upregulate SLC7A11 and downstream molecules such as c-myc and bcl-xl. The circ_0070440/miR-485-5p/SLC7A11 axis is proposed as a potential biomarker axis for clinical detection in LUAD. |
| Confidence score: |
0.8571 |