circRNA basic information
circBase ID: hsa_circ_0006089
Name: hsa_circ_ASAP2
Synonym: circ_0006089
Host Gene: ASAP2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent non-cancerous tissues; tumor tissues
Cell lines:

HGC27; SNU-1; GES-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PTBP3, PI3K, AKT
Associated microRNA: miR-143-3p
Biological function: Promotes GC cell proliferation, migration, invasion, EMT, metastasis, and tumor growth; inhibits apoptosis.
Molecular mechanism: circ_0006089 acts as a ceRNA by sponging miR-143-3p, thereby regulating PTBP3 expression and activating the PI3K/AKT pathway.
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; RNA-seq; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Upregulated circ_0006089 expression was associated with TNM stage III, lymph node metastasis, and lower survival rate; it might serve as a possible prognostic biomarker for GC.
Description:

circ_0006089 is upregulated in gastric cancer tissues and cells and is associated with advanced disease features and poor survival. Functionally, it promotes proliferation, migration, invasion, EMT, metastasis, and tumor growth while suppressing apoptosis. Mechanistically, circ_0006089 sponges miR-143-3p to regulate PTBP3 and activate PI3K/AKT signaling.
Confidence score:

0.8175
Other information
Title:

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Journal: Journal of digestive diseases
Published: 2022
PubMed ID: 35844201
Study type:

combined biological and clinical study
Data availability: GSE83521; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83521
Code availability: -