circRNA basic information
circBase ID: -
Name: -
Synonym: circ100284
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human OS tissue samples; adjacent normal tissue samples; OS tumor tissues
Cell lines:

MG-63 / MG63; U2OS; SAOS-2 / Saos2; HOS; Hfob 1.19
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: lysine-specific histone demethylase 1A (LSD1), enhancer of zeste homolog 2 (EZH2), Phosphatase and tensin homolog (PTEN), epithelial membrane protein 1 (EMP1), H3K27me3, H3K4me2
Associated microRNA: -
Biological function: Circ100284 promotes osteosarcoma cell proliferation, viability, migration, invasion, cell-cycle progression, and is associated with tumor size, WHO grade, lung metastasis, and poorer survival; circ100284 inhibits apoptosis.
Molecular mechanism: Circ100284 directly binds LSD1 and EZH2 and epigenetically silences PTEN and EMP1, negatively regulating PTEN and EMP1 expression to exert its biological functions in osteosarcoma.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Western Blot; Cohort Study; Survival Analysis
Clinical significance:

High circ100284 expression was positively correlated with tumor size, pathological stage, and lung metastasis, and negatively correlated with patient survival time; the circ100284 high-expression group had a poorer survival rate than the low-expression group.
Description:

Circ100284 is up-regulated in human osteosarcoma tissues and cell lines, and high expression is associated with larger tumors, higher WHO grade, lung metastasis, and poorer survival. Functionally, circ100284 promotes OS cell viability, proliferation, migration, invasion, and cell-cycle progression while suppressing apoptosis. Mechanistically, it binds LSD1 and EZH2 and epigenetically represses PTEN and EMP1 expression.
Confidence score:

0.7624
Other information
Title:

Circ100284 promotes invasion and migration of osteosarcoma cells by down-regulating PTEN and EMP1.

Journal: European review for medical and pharmacological sciences
Published: 2020
PubMed ID: 32633341
Study type:

combined biological and clinical study
Data availability: -
Code availability: -