| Expression pattern: |
UP |
| Associated gene: |
CDH3 |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
promotes proliferation and EMT; inhibits apoptosis (overall described as modulating apoptosis) |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism: circ_0023179 sponges miR-615-5p to relieve repression of CDH3, affecting EMT markers and proliferation |
| Biological pathway or process: |
proliferation (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ_0023179 exhibits higher sensitivity and specificity, suggesting its potential as a biomarker for NSCLC. |
| Description: |
circ_0023179 is up-regulated in NSCLC tissues and cell lines and promotes NSCLC cell proliferation and EMT. Mechanistically, it acts as a miRNA sponge for miR-615-5p to de-repress CDH3, and circ_0023179 knockdown suppresses tumor growth in a nude mouse xenograft model. |
| Confidence score: |
0.8134 |