circRNA basic information
circBase ID: hsa_circ_0023404
Name: hsa_circ_RNF121
Synonym: circRNA ring finger protein 121 / circRNF121
Host Gene: RNF121
Genomic location(hg19): chr11:71668272-71671937:+
Genomic location(hg38): chr11:71957226-71960891:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer / CC
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; adjacent normal tissues; tumor samples
Cell lines:

HeLa; CaSki; C33a; End1/E6E7
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ATF2, beta-catenin, c-Myc, cyclinD1
Associated microRNA: miR-153-3p
Biological function: circRNF121 promotes CC cell growth, proliferation, migration, invasion, metastasis, EMT, autophagy, Wnt/beta-catenin pathway activity, and tumor formation; circRNF121 knockdown suppresses these malignant phenotypes.
Molecular mechanism: circRNF121 functions as a sponge of miR-153-3p to regulate ATF2 expression and activate the Wnt/beta-catenin pathway in cervical cancer cells.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); autophagy (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

circRNF121 expression was associated with tumor size, TNM stage, and distant metastasis; circRNF121 might be a novel therapeutic target for cervical cancer.
Description:

circRNF121 is up-regulated in cervical cancer tissues and cell lines and is mainly localized in the cytoplasm. It promotes malignant progression by sponging miR-153-3p, increasing ATF2 expression, and activating the Wnt/beta-catenin pathway, thereby enhancing proliferation, migration, invasion, EMT, autophagy, and tumor growth. Its expression is associated with tumor size, TNM stage, and distant metastasis, suggesting potential therapeutic relevance.
Confidence score:

0.8267
Other information
Title:

CircRNF121 knockdown suppresses the progression of cervical cancer by regulating miR-153-3p/ATF2 axis and wnt/beta-catenin pathway.

Journal: Drug development research
Published: 2022
PubMed ID: 34981843
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -