| Expression pattern: |
UP |
| Associated gene: |
RBPJ, RPBJ, Bax, Bcl-2, cleaved-Caspase-3 |
| Associated microRNA: |
miR-375 |
| Biological function: |
circ-0060428 promotes osteosarcoma cell proliferation and survival, and suppresses apoptosis. |
| Molecular mechanism: |
circ-0060428 acts as a competing endogenous RNA that sponges miR-375, thereby upregulating RBPJ expression and regulating apoptosis-related proteins Bax, Bcl-2, and cleaved-Caspase-3. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-0060428 may serve as a clinically biomarker and treatment target for osteosarcoma therapy. |
| Description: |
This study found that circ-0060428 is up-regulated in osteosarcoma cell lines and is mainly localized in the cytoplasm. Functionally, circ-0060428 promotes osteosarcoma cell proliferation and survival while inhibiting apoptosis. Mechanistically, circ-0060428 sponges miR-375 to upregulate RBPJ expression and modulate apoptosis-related proteins, suggesting it may be a therapeutic target or biomarker for osteosarcoma. |
| Confidence score: |
0.6683 |