| Expression pattern: |
UP |
| Associated gene: |
WNT2, beta-catenin, phosphor beta-catenin, MMP9, E-Cadherin, Vimentin |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
promotes GC cell proliferation, growth, migration, invasion, tumor growth and lung metastasis |
| Molecular mechanism: |
circOSBPL10 acts as a miRNA sponge for miR-136-5p, relieving miR-136-5p-mediated suppression of WNT2 and activating the Wnt/beta-catenin pathway and EMT-related proteins |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circOSBPL10 is positively related to T stage and TNM stage; high circOSBPL10 expression is associated with worse overall survival and disease-free survival; circOSBPL10 may serve as a prognostic factor, potential diagnostic biomarker and therapeutic target in GC |
| Description: |
circOSBPL10 is a human OSBPL10-derived circRNA that is upregulated in gastric cancer tissues and associated with advanced T/TNM stage and poor OS/DFS. Functionally, it promotes GC proliferation, migration, invasion, tumor growth and lung metastasis by sponging miR-136-5p and increasing WNT2 expression, thereby activating Wnt/beta-catenin and EMT-related programs. |
| Confidence score: |
0.8837 |