| Expression pattern: |
DN |
| Associated gene: |
CCNE2, exoribonuclease, Ubiquitin-associated protein 2 |
| Associated microRNA: |
miR-449a |
| Biological function: |
inhibits cellular senescence; promotes proliferation; promotes clonogenicity |
| Molecular mechanism: |
CircCCNB1 acts as a ceRNA/miRNA sponge for miR-449a to regulate CCNE2 expression; CircCCNB1 also binds proteins (CBPs) potentially involved in its degradation. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (not specified) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay |
| Clinical significance: |
- |
| Description: |
CircCCNB1 is down-regulated in senescent human diploid fibroblasts and suppresses cellular senescence. It functions mainly as a ceRNA sponge for miR-449a, thereby relieving repression of CCNE2 and supporting cell cycle/proliferation; it also binds multiple proteins that may influence circCCNB1 stability. |
| Confidence score: |
0.7655 |