| Expression pattern: |
UP |
| Associated gene: |
JMJD2C, beta-catenin, HNRNPK, HNRNPL |
| Associated microRNA: |
miR-205-5p |
| Biological function: |
circ_0000345 promotes CRC cell migration and CRC metastasis; knockdown of circ_0000345 inhibits migration and attenuates JMJD2C/beta-catenin signaling activation, while proliferation is little changed. |
| Molecular mechanism: |
circ_0000345 acts as a sponge for miR-205-5p, impeding miR-205-5p regulation of JMJD2C and enhancing JMJD2C/beta-catenin signaling; HNRNPK and HNRNPL regulate circ_0000345 generation, and kaempferol suppresses this axis by reducing circ_0000345 expression. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); drug resistance (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circ_0000345 shows higher expression in human metastatic CRC tissues than nonmetastatic CRC tissues, suggesting association with CRC metastasis. |
| Description: |
In this study, circ_0000345 is an up-regulated pro-metastatic circRNA in metastatic colorectal cancer. It sponges miR-205-5p to increase JMJD2C expression and activate beta-catenin signaling, thereby promoting CRC migration and metastasis. Kaempferol inhibits CRC metastasis by binding HNRNPK/HNRNPL and reducing circ_0000345-mediated JMJD2C/beta-catenin signaling. |
| Confidence score: |
0.7375 |