| Expression pattern: |
UP |
| Associated gene: |
SPAG5 |
| Associated microRNA: |
miR-320a-3p |
| Biological function: |
promotes proliferation and migration; regulates apoptosis and cell cycle |
| Molecular mechanism: |
ceRNA sponge of miR-320a-3p to regulate SPAG5 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); cell cycle (other); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Clinical Sample Validation; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
The circKNSTRN/miR-320a-3p/SPAG5 axis may serve as a promising therapeutic and prognostic target for BC. |
| Description: |
circKNSTRN (hsa_circ_0103433), derived from KNSTRN, is up-regulated in breast cancer tissues and cell lines and predominantly localizes in the cytoplasm. It promotes breast cancer cell proliferation and migration, and modulates apoptosis and the cell cycle, mainly by sponging miR-320a-3p to increase SPAG5 expression via a ceRNA (circKNSTRN/miR-320a-3p/SPAG5) axis. |
| Confidence score: |
0.8625 |