| Expression pattern: |
UP |
| Associated gene: |
MCTS1, IL-6 |
| Associated microRNA: |
miR-889-3p |
| Biological function: |
promotes proliferation and migration; promotes malignant progression |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-889-3p, relieving repression of MCTS1 and promoting IL-6 secretion. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; ELISA; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression of circHIPK2 may predict the poor prognosis of LSCC; potential diagnostic and prognostic biomarker for LSCC. |
| Description: |
circHIPK2 is up-regulated in LSCC tissues and cell lines and promotes proliferation and migration. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-889-3p, thereby increasing MCTS1 expression and enhancing IL-6 secretion to accelerate malignant progression, with potential diagnostic/prognostic value. |
| Confidence score: |
0.8175 |