| Expression pattern: |
DN |
| Associated gene: |
PDZD2, IGF2BP2 |
| Associated microRNA: |
miR-154-3p |
| Biological function: |
circ_0000009 acts as a tumor suppressor in LUAD; its overexpression suppresses LUAD cell proliferation and tumor growth and promotes apoptosis. |
| Molecular mechanism: |
circ_0000009 upregulates PDZD2 through a ceRNA mechanism by sponging miR-154-3p and stabilizes PDZD2 by recruiting the RBP IGF2BP2. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other); mRNA stability (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down |
| Clinical significance: |
circ_0000009 might be considered as a new diagnostic and therapeutic target for LUAD. |
| Description: |
hsa_circ_0000009 (circ_0000009) is downregulated in LUAD tissues and cell lines and functions as a tumor suppressor. Its overexpression inhibits LUAD cell proliferation and xenograft tumor growth while promoting apoptosis, through upregulating PDZD2 via miR-154-3p sponging and IGF2BP2-mediated PDZD2 mRNA stabilization. |
| Confidence score: |
0.8275 |