| Expression pattern: |
UP |
| Associated gene: |
ETV1, Ago2 |
| Associated microRNA: |
miR-22-3p |
| Biological function: |
Promotes RCC cell growth, motility (migration), invasion, xenograft tumor growth, and M2-type macrophage polarization; circPRELID2 silencing inhibits these malignant phenotypes. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge: circPRELID2 sequesters miR-22-3p, thereby increasing ETV1 expression; the miR-22-3p/ETV1 axis mediates circPRELID2 effects on malignant behaviors. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); macrophage polarization (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Microarray; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High circPRELID2 expression was closely linked to tumor lymph node metastasis and TNM stage, suggesting potential as a biomarker for RCC diagnosis and progression. |
| Description: |
circPRELID2 is up-regulated in renal cell carcinoma and promotes malignant phenotypes (proliferation, migration/motility, invasion) and xenograft tumor growth. Mechanistically, it acts as a miR-22-3p sponge to upregulate ETV1, and its knockdown also weakens M2-type macrophage polarization. |
| Confidence score: |
0.8635 |