| Expression pattern: |
UP |
| Associated gene: |
MYBL2, E-cadherin, Vimentin, CCNB1, P21, DICER |
| Associated microRNA: |
miR-143 |
| Biological function: |
promotes proliferation, colony formation, invasion, EMT and tumor growth of bladder cancer |
| Molecular mechanism: |
acts as a miRNA sponge for miR-143 to increase MYBL2 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); EMT (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; divergent primers PCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; ROC Analysis; Clinical Sample Validation |
| Clinical significance: |
High expression correlated with tumor-node-metastasis (TNM) stage and muscular invasion; potential early diagnostic marker (AUC 0.860). |
| Description: |
circ_0006332 is up-regulated in bladder cancer tissues and mainly localizes in the cytoplasm. It promotes bladder cancer proliferation, invasion, EMT, and in vivo xenograft growth by acting as a ceRNA sponge for miR-143, thereby increasing MYBL2 expression; it also shows diagnostic potential (ROC AUC reported). |
| Confidence score: |
0.8279 |