circRNA basic information
circBase ID: hsa_circ_0001445
Name: hsa_circ_SMARCA5
Synonym: cSMARCA5
Host Gene: SMARCA5
Genomic location(hg19): chr4:144464661-144465125:+
Genomic location(hg38): chr4:143543508-143543972:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent noncancerous liver tissues; healthy liver tissues
Cell lines:

SMMC-7721; HCCLM3; Huh7; Hep3B; MHCC97H; HEK-293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: TIMP3, DHX9, AGO2
Associated microRNA: miR-17-3p, miR-181b-5p
Biological function: Inhibits HCC growth, migration and metastasis in vitro and in vivo; acts as a tumor suppressor; lower expression is associated with aggressive clinicopathological characteristics and poor prognosis.
Molecular mechanism: DHX9 upregulation inhibits cSMARCA5 biogenesis; cSMARCA5 functions as a sponge for miR-17-3p and miR-181b-5p to upregulate TIMP3 and inhibit HCC growth and metastasis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); metastasis (inhibits); ceRNA regulation (promotes); other pathway/process (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; Northern Blot; FISH / smFISH; IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis; RNA-seq
Clinical significance:

Downregulated cSMARCA5 is associated with aggressive clinicopathological characteristics, poorer overall survival and recurrence-free survival, and can serve as an independent prognostic marker after hepatectomy.
Description:

cSMARCA5 (hsa_circ_0001445), derived from SMARCA5 exons 15 and 16, is significantly downregulated in HCC and its low expression predicts aggressive disease and poor survival after hepatectomy. Functionally, cSMARCA5 suppresses HCC cell growth, migration and metastasis in vitro and in xenograft models. Mechanistically, DHX9 upregulation contributes to cSMARCA5 downregulation, while cSMARCA5 sponges miR-17-3p and miR-181b-5p to upregulate the tumor suppressor TIMP3.
Confidence score:

0.9057
Other information
Title:

Circular RNA cSMARCA5 inhibits growth and metastasis in hepatocellular carcinoma.

Journal: Journal of hepatology
Published: 2018
PubMed ID: 29378234
Study type:

combined biological and clinical study
Data availability: GSE14520; GSE76903; GSM2258636; Table S4; Table S5; Table S9; Supplementary data at https://doi.org/10.1016/j.jhep.2018.01.012
Code availability: -