circRNA basic information
circBase ID: hsa_circ_0001178
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr21:17135209-17138460:+
Genomic location(hg38): chr21:15762890-15766141:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissue specimens; lung tissues; liver tissues
Cell lines:

HCT116; HT29; HCT8; SW480; LoVo; SW620; NCM460; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ZEB1
Associated microRNA: miR-382, miR-587, miR-616
Biological function: Promotes CRC cell migration, invasion, EMT, lung and liver metastasis, and metastatic dissemination.
Molecular mechanism: hsa_circ_0001178 acts as a cytoplasmic miRNA sponge for miR-382, miR-587 and miR-616, upregulates ZEB1, induces EMT, and forms a positive feedback loop because ZEB1 transcriptionally activates hsa_circ_0001178.
Biological pathway or process:

ceRNA regulation (promotes); EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; IHC (Immunohistochemistry); Luciferase Reporter Assay; ChIP / ChIP-seq; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High hsa_circ_0001178 expression was positively correlated with lymph node metastasis, distant metastasis, advanced TNM stage, shorter overall survival and shorter distant metastasis-free survival; it may be a promising prognostic indicator for CRC.
Description:

hsa_circ_0001178 is upregulated in metastatic colorectal cancer and high expression is associated with aggressive clinicopathologic features and poor survival. Functionally, it promotes CRC migration, invasion, EMT, and lung/liver metastasis by sponging miR-382, miR-587, and miR-616 to increase ZEB1 expression. ZEB1 also transcriptionally activates hsa_circ_0001178, forming a positive feedback ceRNA loop that amplifies CRC metastatic dissemination.
Confidence score:

0.7789
Other information
Title:

Circular RNA hsa_circ_0001178 facilitates the invasion and metastasis of colorectal cancer through upregulating ZEB1 via sponging multiple miRNAs.

Journal: Biological chemistry
Published: 2020
PubMed ID: 31747371
Study type:

combined biological and clinical study
Data availability: -
Code availability: -