| Expression pattern: |
DN |
| Associated gene: |
HNRNPD |
| Associated microRNA: |
- |
| Biological function: |
not specified |
| Molecular mechanism: |
HNRNPD suppresses circCDK1 biogenesis and shifts the linear splicing/backsplicing balance toward CDK1 mRNA, thereby affecting CDK1 mRNA/protein levels linked to cell cycle and apoptosis phenotypes; circCDK1 itself showed no direct effect on cell cycle/apoptosis in tested assays. |
| Biological pathway or process: |
cell cycle (other); apoptosis (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; circRNA-seq; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circCDK1 is lower in ccRCC specimens than in para-ccRCC specimens, and the circCDK1:CDK1 mRNA ratio is lower in ccRCC tumour tissues than in para-ccRCC tissues. |
| Description: |
In ccRCC, circCDK1 is decreased in tumor tissues, while HNRNPD modulates the linear splicing/backsplicing balance of the CDK1 locus. Altered circCDK1:CDK1 mRNA ratio reflects HNRNPD-dependent circRNA biogenesis control; circCDK1 itself showed no direct effect on cell cycle/apoptosis in functional assays reported. |
| Confidence score: |
0.7373 |