circRNA basic information
circBase ID: hsa_circ_0010235
Name: hsa_circ_ALDH4A1
Synonym: circ_0010235
Host Gene: ALDH4A1
Genomic location(hg19): chr1:19201875-19216599:-
Genomic location(hg38): chr1:18875381-18890105:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; surrounding normal tissues; adjacent normal tissues; xenograft tumors

Cell lines:

A549; H1299; HBE; A549/PTX; H1299/PTX; Human umbilical vein endothelial cells

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: FAM83F, Bax, Bcl-2, P-gp
Associated microRNA: miR-512-5p
Biological function: circ_0010235 promotes paclitaxel resistance, proliferation, angiogenesis, migration and invasion of PTX-resistant NSCLC cells and suppresses apoptosis; circ_0010235 knockdown enhances PTX-induced cytotoxicity and antitumor sensitivity.
Molecular mechanism: circ_0010235 acts as a ceRNA by sponging miR-512-5p to positively regulate FAM83F expression, thereby stimulating paclitaxel resistance in NSCLC.
Biological pathway or process:

drug resistance (promotes); chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis

Clinical significance:

circ_0010235/miR-512-5p/FAM83F axis may be a potential therapeutic option for patients with NSCLC who are resistant to PTX.

Description:

circ_0010235 is upregulated in NSCLC and especially in PTX-resistant NSCLC tissues and cells. It promotes paclitaxel resistance and malignant phenotypes by sponging miR-512-5p to increase FAM83F expression, while knockdown of circ_0010235 improves PTX sensitivity in vitro and in a xenograft model.

Confidence score:

0.7859

Other information
Title:

Blocking circ_0010235 suppresses acquired paclitaxel resistance of non-small cell lung cancer by sponging miR-512-5p to modulate FAM83F expression.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 36206095
Study type:

combined biological and clinical study

Data availability: -
Code availability: -